Candida parapsilosis as VERO cell culture contaminant in Neospora caninum antigen production/Candida parapsilosis como contaminante de cultura de celulas VERO na producao de antigeno de Neospora caninum/Candida parapsilosis como contaminante de cultivos celulares VERO en la produccion de Neospora caninum antigen

Neospora caninum has been identified as an important cause of infectious in cattle and dogs. The parasite is successfully established in continuous passage in VERO cell cultures. Cell culture contamination may present itself in two mainly forms: contamination with other cell line or with microorgani...

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Published inVeterinária e zootecnia Vol. 20; no. 1; p. 62
Main Authors Cavalcante, Raissa Vasconcelos, Kikuti, Mariana, Richini-Pereira, Virginia Bodelao, Langoni, Helio
Format Journal Article
LanguagePortuguese
Published Universidade Estadual Paulista. Facultade de Medicina Veterinaria e Zootecnia 01.03.2013
Subjects
Antigens
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Summary:Neospora caninum has been identified as an important cause of infectious in cattle and dogs. The parasite is successfully established in continuous passage in VERO cell cultures. Cell culture contamination may present itself in two mainly forms: contamination with other cell line or with microorganisms. In this paper we report a VERO cell culture of Neospora caninum antigen production contaminated with Candida parapsilosis. Sequencing of the ITS amplicons showed 99% similarity with deposited sequences of Candida parapsilosis at GenBank. It should be noted through this report the possibility of contamination by Candida parapsilosis in cell cultures, thus care in handling cultures should be redoubled.
ISSN:0102-5716
0102-5716