Overexpression of ITaMYB4/I Confers Freezing Tolerance in IArabidopsis thaliana/I

Freezing stress is one of the main factors limiting the growth and yield of wheat. In this study, we found that TaMYB4 expression was significantly upregulated in the tillering nodes of the strong cold-resistant winter wheat variety Dongnongdongmai1 (Dn1) under freezing stress. Weighted gene co-expr...

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Published inInternational journal of molecular sciences Vol. 24; no. 13
Main Authors Tian, Yu, Peng, Kankan, Ma, Xuan, Ren, Zhipeng, Lou, Guicheng, Jiang, Yunshuang, Xia, Jingqiu, Wang, Duojia, Yu, Jing, Cang, Jing
Format Journal Article
LanguageEnglish
Published MDPI AG 01.07.2023
Subjects
Analysis
Arabidopsis thaliana
Cang, Joel
Genes
Genetic engineering
Wheat
China
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Summary:Freezing stress is one of the main factors limiting the growth and yield of wheat. In this study, we found that TaMYB4 expression was significantly upregulated in the tillering nodes of the strong cold-resistant winter wheat variety Dongnongdongmai1 (Dn1) under freezing stress. Weighted gene co-expression network analysis, qRT-PCR and protein–DNA interaction experiments demonstrated that monodehydroascorbate reductase (TaMDHAR) is a direct target of TaMYB4. The results showed that overexpression of TaMYB4 enhanced the freezing tolerance of transgenic Arabidopsis. In TaMYB4 overexpression lines (OE-TaMYB4), AtMDHAR2 expression was upregulated and ascorbate-glutathione (AsA–GSH) cycle operation was enhanced. In addition, the expression of cold stress marker genes such as AtCBF1, AtCBF2, AtCBF3, AtCOR15A, AtCOR47, AtKIN1 and AtRD29A in OE-TaMYB4 lines was significantly upregulated. Therefore, TaMYB4 may increase freezing tolerance as a transcription factor (TF) in Arabidopsis through the AsA–GSH cycle and DREB/CBF signaling pathway. This study provides a potential gene for molecular breeding against freezing stress.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms241311090