ICcNAC1/I by Transcriptome Analysis Is Involved in Sudan Grass Secondary Cell Wall Formation as a Positive Regulator

• Published in: International journal of molecular sciences Vol. 24; no. 7
• Main Authors: Huang, Yanzhong, Qian, Chen, Lin, Jianyu, Antwi-Boasiako, Augustine, Wu, Juanzi, Liu, Zhiwei, Mao, Zhengfeng, Zhong, Xiaoxian
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.03.2023
• Subjects: Analysis; Arabidopsis thaliana; Genetic transcription; Lignin; Metabolites; Proteins; China; Sudan
• ISSN: 1422-0067; 1422-0067
• DOI: 10.3390/ijms24076149

Sudan grass is a high-quality forage of sorghum. The degree of lignification of Sudan grass is the main factor affecting its digestibility in ruminants such as cattle and sheep. Almost all lignocellulose in Sudan grass is stored in the secondary cell wall, but the mechanism and synthesis of the secondary cell wall in Sudan grass is still unclear. In order to study the mechanism of secondary cell wall synthesis in Sudan grass, we used an in vitro induction system of Sudan grass secondary cell wall. Through transcriptome sequencing, it was found that the NAC transcription factor CcNAC1 gene was related to the synthesis of the Sudan grass secondary cell wall. This study further generated CcNAC1 overexpression lines of Arabidopsis to study CcNAC1 gene function in secondary cell wall synthesis. It was shown that the overexpression of the CcNAC1 gene can significantly increase lignin content in Arabidopsis lines. Through subcellular localization analysis, CcNAC1 genes could be expressed in the nucleus of a plant. In addition, we used yeast two-hybrid screening to find 26 proteins interacting with CcNAC1. GO and KEGG analysis showed that CcNAC1 relates to the metabolic pathways and biosynthesis of secondary metabolites. In summary, the synthesis of secondary cell wall of Sudan grass can be regulated by CcNAC1.