Synthesis of silica nanoparticles doped with [Ru ions

A sensitive and selective luminescent nanoprobe (referred to as DEPN) is designed for the determination of Cu(II) ions. DEPN shows two emission peaks, one at 602 nm and caused by [Ru(bpy).sub.3].sup.2+, the other (peaking at 500 nm) caused by silver nanoclusters (AgNCs). The luminescence of the ruth...

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Published inMikrochimica acta (1966) Vol. 184; no. 7; p. 2325
Main Authors Huang, Xinan, Jiang, Hong, Li, Yongxin, Sang, Lijia, Zhou, Huipeng, Shahzad, Sohail Anjum, Ibupoto, Zafar Hussain
Format Journal Article
LanguageEnglish
Published Springer 01.07.2017
Subjects
Ethylenediaminetetraacetic acid
Fluorescence microscopy
Silica
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Summary:A sensitive and selective luminescent nanoprobe (referred to as DEPN) is designed for the determination of Cu(II) ions. DEPN shows two emission peaks, one at 602 nm and caused by [Ru(bpy).sub.3].sup.2+, the other (peaking at 500 nm) caused by silver nanoclusters (AgNCs). The luminescence of the ruthenium complex is inert and acts as the internal reference signal, while that of the AgNCs is quenched by Cu(II) ions. Under 350 nm photoexcitation, a linear relationship is found between the ratio of emission intensity (I.sub.500/I.sub.602) and the concentration of Cu(II) ions in the 0.1-16 [mu]M range. The detection limit of Cu(II) ions is estimated to be 50 nM. The DEPN was applied to the determination of Cu(II) ions in (spiked) water samples, and to intracellular imaging of Cu(II) in HeLa cells using confocal fluorescence microscopy.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-017-2206-3