Enumeration of Campylobacter spp. and presence of Campylobacter jejuni in broiler carcasses in the State of Minas Gerais, Brazil/Enumeracao de Campylobacter spp. e presenca de Campylobacter jejuni em carcacas de frango no Estado de Minas Gerais

• Published in: Ciência rural Vol. 43; no. 3; p. 480
• Main Authors: Oliveira, Afonso de Liguori, de Oliveira, Roseane Batitucci Passos
• Format: Journal Article
• Language: Spanish
• Published: Universidade Federal de Santa Maria 01.03.2013
• Subjects: Dressed poultry; Poultry industry; Zoonoses
• ISSN: 0103-8478; 1678-4596

Campylobacteriosis is a worldwide distribution zoonosis, with significant repercussions on public health and with a high socioeconomic impact. Routes of transmission of Campylobacter species to man are direct contact with carrier animals, contaminated water or food and it is most commonly associated with consumption of chicken meat. The objective of this study was to detect and enumerate the Campylobacter spp. by a rapid method (SimPlate[R]) and and conventional method (direct plating on mCCDA) and determine frequency and level of contamination in 75 broiler carcasses (25 pre-chill; 25 post-chill at establishments under federal inspection in the State of Minas Gerais, Brazil and 25 frozen broiler carcass samples taken from the retail trade). Evaluated samples, regardless of the technique used, detected the presence of Campylobacter spp. in 56.0% before the chiller and 44.0% after the chiller. Counts of the frozen samples were below the detection limit of the method. Of the 75 samples tested, 34.7% were positive for Campylobacter spp. and that, 38.5% were identified as C. jejuni. Among them, the largest number of C. jejuni obtained was from the carcass of the chicken before the chiller (42.9%), followed by chicken carcass after the chiller (36.4%). The study showed that the detection and enumeration is variable, although in none of the samples intended for trade has been obtained scores above the infective dose. The use of SimPlate[R] technique was appropriate and equivalent to direct plating on agar mCCD and showed a better recovery rate cells of Campylobacter spp.. Key words: broiler meat, Campylobacter spp., Campylobacter jejuni, identification, contamination. Campilobacteriose e uma zoonose de distribuicao mundial, com repercussoes importantes na saude publica e um grande impacto socioeconomico. As rotas de transmissao ao homem das especies de Campylobacter sao o contato direto com animais portadores, agua ou alimentos contaminados e e mais comumente associado ao consumo de carne de frango. O objetivo deste estudo foi detectar e enumerar Campylobacter spp. por um metodo rapido (SimPlate[R]) e pelo metodo convencional (plaqueamento direto em agar mCCD), determinando a frequencia e o nivel de contaminacao em 75 amostras de carcacas de frangos (25 Pre-chiller e 25 Poschiller obtidos em abatedouros sob inspecao federal no Estado de Minas Gerais e 25 amostras congeladas, coletadas no comercio verejista). As amostras avaliadas, independentemente do metodo utilizado, apresentaram Campylobacter spp. em 56,0% das carcacas antes do chiller e 44,0% apos o chiller. A contagem das amostras congeladas se apresentou abaixo do limite de deteccao do metodo. Das 75 amostras testadas, 34,7% foram positivas para Campylobacter spp. Das amostras positivas, 38,5% foram identificadas como C. jejuni e, entre estas, o maior numero de C. jejuni foi obtido nas carcacas de frango antes do chiller (42,9%), seguido de carcaca de frango apos o chiller (36,4%). O estudo mostrou que a deteccao e enumeracao e variavel, embora em nenhuma das amostras destinadas ao comercio foram obtidas contagens acima da dose infectante estabelecida. O uso do SimPlate[R] foi adequado e equivalente ao plaqueamento direto em agar mCCD e apresentou uma melhor taxa de recuperacao de celulas de Campylobacter spp. Palavras-chave: carne de frango, Campylobacter spp., Campylobacter jejuni, identificacao, contaminacao.