The influence of membrane composition on the release of polyphenols from liposomes/Uticaj sastava membrane na brzinu otpustanja polifenola iz lipidnih mikrocestica

• Published in: Hemijska industrija p. 347
• Main Authors: Pravilovic, Radoslava N, Radunovic, Vesna S, Boskovic-Vragolovic, Nevenka M, Bugarski, Branko M, Pjanovic, Rada V
• Format: Journal Article
• Language: English
• Published: Association of Chemical Engineers of Serbia 01.07.2015
• Subjects: Analysis; Chemical properties; Health aspects; Liposomes; Polyphenols; Serbia
• ISSN: 0367-598X; 2217-7426
• DOI: 10.2298/HEMIND140220060P

Polyphenols are compounds which are widely studied due to their antioxidative and potential therapeutic properties. Systems for the controlled release of drugs offer a number of benefits comparing with traditional forms of medicines and because of that these systems are widely researched. The objective of this paper is to investigate the possibility of using liposomes as carriers of polyphenols and influence of the membrane composition on the release rate of encapsulated polyphenols. Experiments show how the membrane modification affects the mass transfer comparing to a conventional liposomes. Liposomes were modified with surfactants Tween 20 and Tween 60, and thyme tea extract was used as a source of polyphenols. The diffusion of polyphenols from thyme extract, dispersion of conventional liposomes and liposomes modified with Tween 20 and Tween 60 were studied using Franz diffusion cell. From the experimental data diffusion coefficients were determined for each of the systems, as well as the corresponding diffusion resistances. From the obtained results it can be concluded that the encapsulation of polyphenols in liposomes significantly slows diffusion, and with membrane modification can be achieved further slowing. The diffusion resistance of the liposome membrane modified with Tween 20 and Tween 60 is about 5 times higher compared to the diffusion of unencapsulated polyphenols from the thyme extract. Keywords: diffusion coefficient, diffusion resistance, liposomes, membrane modification, polysorbate, tea polyphenols. Available online at the Journal website: Sistemi za kontrolisano otpustanje lekova predstavljaju istrazivacku oblast koja se intenzivno proucava zbog niza prednosti koje ovi sistemi pruzaju i moguceg unapredenja dosadasnjih, tradicionalnih formi lekova. Fosfolipidne mikrocestice (lipozomi) su se pokazale potencijalno pogodnim nosacima aktivnih supstanci u sistemima ovog tipa. Predmet ovog rada je ispitivanje uticaja sastava membrane lipozoma na brzinu oslobadanja inkapsuliranih polifenola. Eksperimentalna ispitivanja su pokazala kako modifikacija membrane lipozoma utice na prenos mase, u odnosu na konvencionalne lipozome. Lipozomi su modifikovani povrsinski aktivnim materijama Tween 20 i Tween 60, dok je kao izvor polifenola koriscen cajni ekstrakt biljke majcine dusice. Eksperimenti su izvedeni koriscenjem Franz-ove difuzione celije, u kojima je pracena difuzija polifenola iz: cajnog ekstrakta, disperzije konvencionalnih lipozoma, disperzije lipozoma modifikovanih pomocu Tween 20 i disperzije lipozoma modifikovanih pomocu Tween 60. Obradom eksperimentalnih rezultata odredeni su koeficijenti difuzije za svaki od sistema, kao i odgovarajuci difuzioni otpori. Na osnovu dobijenih rezultata moze se zakljuciti da se inkapsulacijom polifenola u fosfolipidne mikrocestice-lipozome, znacajno usporava njihova difuzija, a da se modifikacijom membrane moze postici dodatno usporavanje prenosa mase, sto zavisi od strukture molekula kojim se modifikacija vrsi. Molekuli Tween 20 kao i Tween 60 su se pokazali uspesnim modifikatorima membrane lipozoma. Otpori koje membrane modifikovane navedenim polisorbatima pruzaju difuziji polifenola su veci i dovode do 4,9 puta sporije difuzije u odnosu na sistem sa neinkapsuliranim polifenolima. Kljucne reci: Koeficijent difuzije * Difuzioni otpor * Lipozomi * Modifikacija membrane * Polisorbati * Polifenoli