Histometric study of alveolar bone in rats submitted to ethanol during lactation/ Estudio histometrico del hueso alveolar en ratas sometidas a etanol durante la lactancia

The present work studied the adverse effects of maternal exposure of rats to alcohol during lactation, on the development of their offspring. Histometric evaluation by karyometry and of the alveolar bone at the level of the first upper molar of the sucking was performed. Two groups of animals, one c...

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Bibliographic Details
Published inInternational journal of morphology Vol. 26; no. 4; p. 945
Main Authors Curi, Viviane, Azoubel, Reinaldo, Lopes, Ruberval Armando, Ma, Di Matteo, Miguel Angel Sala, Kassis, Elias Naim
Format Journal Article
LanguageSpanish
Published Universidad de La Frontera, Facultad de Medicina 01.12.2008
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Summary:The present work studied the adverse effects of maternal exposure of rats to alcohol during lactation, on the development of their offspring. Histometric evaluation by karyometry and of the alveolar bone at the level of the first upper molar of the sucking was performed. Two groups of animals, one coming from mothers exposed to drinking water containing 20% ethanol during the total lactation period and the other of controls coming from mothers receiving only alcohol-free drinking water during this period. On the 21 first day of lactation the young of each group were aleatorily selected and following anesthesia, their heads severed; after histological treatment, serial 6 [micro] m sections on the frontal plane at the molar level, stained with hematoxilin and eosin, were obtained. The experimental results produced, suggest that sucking from ethanol-treated mothers, show retarded post-natal growth, their alveolar bones presenting scarce, little calcified trabeculae, and a more abundant bone marrow compared to controls. KEY WORDS: Ethanol; Alveolar bone; Lactation. Considerando que la lactancia es un periodo importante y vulnerable, y que la exposicion materna al alcohol durante esta fase puede provocar efectos adversos en las crias en desarrollo, el proposito del presente trabajo es evaluar histometricamente (mediante cariometria y estereologia), el hueso alveolar a nivel del primer molar superior, de ratas lactantes sometidas a los efectos del etanol. Con esa finalidad utilizamos dos grupos, uno que recibio etanol al 20% en el bebedero, durante todo el periodo de lactancia (21dias), y otro que recibio solo agua. En el 21 dia de lactancia, 5 ratas de cada grupo fueron aleatoriamente seleccionadas. Despues de sacrificados, los animales fueron fijados en formol al 10%. Las cabezas, separadas de los cuerpos, fueron incluidas en parafina, y cortadas seriadamente con 6 [micron] m de grosor, a partir de un plano frontal al nivel de los molares, y tenidas con hematoxilina y eosina. Basados en los resultados presentes, es posible concluir que el etanol al 20% en el agua de beber, administrado a ratas lactantes, provoca los siguientes efectos: reduccion significativa del peso corporal, alteraciones de las trabeculas del hueso alveolar, observadas estereologicamente, evidenciadas por trabeculas delicadas y poco calcificadas, con medula abundante, nucleos menores en osteoblastos y osteocitos y mas achatados en los primeros, y con osteocitos de volumenes citoplasmatico y celular menor y densidad numerica mayor. PALABRAS CLAVE: Etanol; Hueso Alveolar; Lactato.
ISSN:0717-9367
0717-9502