Morphoquantitative analysis of the intraepithelial neoplasic uterine cells by computer image system/Analisis morfocuantitativo de las celulas neoplasicas intraepiteliales uterinas por sistema computarizado

The purpose of the present research was to detect the DNA variations by Feulgen stained chromatin and the packing degree of the cervical chromatin in the clinical cases of human uterine carcinomas. Scanning microphotometry was carried out on slides prepared from block Feulgen-stained. Accurate contr...

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Published inInternational journal of morphology Vol. 26; no. 1; p. 93
Main Authors Lima, Jacqueline Silva Brito, Bittencourt, Alexandre Motta, Lima, Carlos Eduardo de Queiroz, da Mota, Diogenes Luis, Romaguera, Ana Maria de Ataide, da Silva, Luiz Lucio Soares
Format Journal Article
LanguageSpanish
Published Universidad de La Frontera, Facultad de Medicina 01.03.2008
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Summary:The purpose of the present research was to detect the DNA variations by Feulgen stained chromatin and the packing degree of the cervical chromatin in the clinical cases of human uterine carcinomas. Scanning microphotometry was carried out on slides prepared from block Feulgen-stained. Accurate control procedures were performed using normal human cell nuclei. The cervical cells was morphometric studied using the Image Lab System for citologic classification. The overall diagnostic accuracy for neoplasia type I (CIN I) and type II (CIN II), demonstrated by stain interpetration values of a sensitive detection of DNA neuroploidy and a significant difference between the two groups in scanning static cytophotometry. KEY WORDS: Cervix; DNA; Cervical Cancer; Morphometry. La investigacion tuvo como objetivo detectar las posibles variaciones del DNA en el nucleo de las celulas marcadas por la reaccion histoquimica de Feulgen en individuos con diagnostico de neoplasias intraepiteliales de grados I y II del cuello uterino. La microcitometria fue realizada en laminas provenientes de hospitales universitarios y estatales, sometidas a reaccion de Feulgen y analizadas en el sistema de imagen (Image Lab) para cuantificacion. Los resultados encontrados entre las celulas neoplasicas de grado I (NIC I) y del grado II (NIC II) demostraron que el metodo es sensible y eficiente en la cuantificacion entre las diferentes clasificaciones, ayudando asi en los diagnosticos de las lesiones del cuello uterino en su fase inicial. PALABRAS CLAVE: Cuello Uterino; DNA; Cancer Cervical; Morfometria.
ISSN:0717-9367
0717-9502