Transgenic poplar

Poplar is a valuable tree species that is distributed all over the world. However, many insect pests infest poplar trees and have caused significant damage. To control poplar pests, we transformed a poplar species, Populus davidiana x P bolleana Loucne, with the dsRNA of the chitinase gene of a popl...

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Bibliographic Details
Published inJournal of insect science (Tucson, Ariz.) Vol. 24; no. 4
Main Authors Jiang, Yun-Xiao, Li, Man-Yu, Han, Qing, Tan, Jia-Lin, Wang, Zi-Yan, Jing, Tian-Zhong
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.07.2024
Subjects
Botanical research
Chitin
Diseases and pests
Genetic aspects
Identification and classification
Physiological aspects
Phytophagous insects
Plant genetics
Plant immunology
Poplar
RNA
Turkey
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Summary:Poplar is a valuable tree species that is distributed all over the world. However, many insect pests infest poplar trees and have caused significant damage. To control poplar pests, we transformed a poplar species, Populus davidiana x P bolleana Loucne, with the dsRNA of the chitinase gene of a poplar defoliator, Clostera anastomosis (Linnaeus) (Lepidoptera: Notodontidae), employing an Agrobaterium-mediated approach. The transgenic plant has been identified by cloning the T-DNA flanking sequences using TAIL-PCR and quantifying the expression of the dsRNA using qPCR. The toxicity assay of the transgenic poplar lines was carried out by feeding the target insect species (C. anastomosis). The results showed that, in C. anastomosis, the activity of chitinase was significantly decreased, consistent with the expression on mRNA levels, and the larval mortality was significantly increased. These results suggested that the transgenic poplar of dsRNA could be used for pest control.
ISSN:1536-2442
1536-2442
DOI:10.1093/jisesa/ieae087