The role of copper chromite nanoparticles on physical and bio properties of scaffolds based on poly

Tissue engineering combines suitable cells, engineering methods, and proper biochemical factors to develop functional and biological tissues and repair damaged tissues. In this study, we focused on synthesizing and characterizing a nanocomposite scaffold based on glycerol and azelaic acid (Gl-Az) co...

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Published inCell and tissue research Vol. 391; no. 2; p. 357
Main Authors Shams, Mehdi, Salimi, Ali, Goodarzi, Vahabodin, Kazemi, Negar Motakef, Heydari, Masoumeh
Format Journal Article
LanguageEnglish
Published Springer 01.02.2023
Subjects
Glycerin
Glycerol
Nanoparticles
Spinel group
Stem cells
Tissue engineering
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Summary:Tissue engineering combines suitable cells, engineering methods, and proper biochemical factors to develop functional and biological tissues and repair damaged tissues. In this study, we focused on synthesizing and characterizing a nanocomposite scaffold based on glycerol and azelaic acid (Gl-Az) combined with copper chromite (CuCr.sub.2O.sub.4) nanoparticles in order to increase the osteogenic differentiation efficiency of human adipose-derived stem cells (hADSCs) on fabricated scaffolds. The degradability and hydrophobicity properties as well as mechanical and thermal behaviors of nanocomposite scaffolds were investigated. Next, the cell toxicity of glycerol, azelaic acid and CuCr.sub.2O.sub.4 nanoparticles was studied by MTT assay test and acridine orange staining. Finally, the osteogenic differentiation of hADSCs on Gl-Az-CuCr.sub.2O.sub.4 scaffolds was examined using alkaline phosphatase activity (ALP) and calcium content. The obtained results demonstrated that Gl-Az-1%CuCr.sub.2O.sub.4 not only showed appropriate mechanical strength, biocompatibility and degradability but also influenced the capability of hADSCs to differentiate into osteogenic lineages. The hADSCs culture in Gl-Az-1%CuCr.sub.2O.sub.4 showed a significant increase in ALP activity levels and calcium biomineralization after 14 days of osteogenic differentiation. In conclusion, the Gl-Az-1%CuCr.sub.2O.sub.4 nanocomposite could be used as a biocompatible and degradable scaffold to induce the bone differentiation of hADSCs and it could be a promising scaffold in bone regenerative medicine.
ISSN:0302-766X