Development of a rapid and reliable single-tube multiplex real-time PCR method for

is significantly associated with cutaneous adverse drug reactions caused by aromatic antiepileptic drugs. Here, we aimed to establish a fast and reliable detection method for genotyping. A single-tube multiplex quantitative real-time polymerase chain reaction (qPCR) assay for genotyping was establis...

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Bibliographic Details
Published inPharmacogenomics Vol. 20; no. 11; pp. 803 - 812
Format Journal Article
LanguageEnglish
Published Future Medicine Ltd 01.08.2019
01.07.2019
01.06.2019
Subjects
ARMS
aromatic antiepileptic drugs (AEDs)
cutaneous adverse drug reactions (cADRs)
qPCR
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Summary:is significantly associated with cutaneous adverse drug reactions caused by aromatic antiepileptic drugs. Here, we aimed to establish a fast and reliable detection method for genotyping. A single-tube multiplex quantitative real-time polymerase chain reaction (qPCR) assay for genotyping was established by combining allele-specific primers with TaqMan probes. A 100% concordance was observed between qPCR and SBT result in 106 Han subjects. The detection limit of the new method was 0.05 ng DNA. The positive rate of in Tibetans (55.6%, n = 81) was significantly higher than those in Han (34%, n = 106), Uighur (27.5%, n = 102), Bouyei (25.9%, n = 116) and Miao populations (26.5%, n = 113). The newly established qPCR assay was reliable for screening in clinical applications.
ISSN:1462-2416
1744-8042
DOI:10.2217/pgs-2019-0052