Evaluation of predictive

To align predicted and measured CYP2C19 phenotype in a South African cohort. Genotyping of and was performed using PCR-RFLP, and an activity score (AS) system was used to predict phenotype. True phenotype was measured using plasma concentrations of omeprazole and its metabolite 5′-hydroxyomperazole....

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Published inPharmacogenomics Vol. 16; no. 12; pp. 1343 - 1354
Main Authors Dodgen, Tyren M, Drögemöller, Britt I, Wright, Galen EB, Warnich, Louise, Steffens, Francois E, Cromarty, A Duncan, Alessandrini, Marco, Pepper, Michael S
Format Journal Article
LanguageEnglish
Published Future Medicine Ltd 01.08.2015
Subjects
activity score system
genotype-phenotype correlation
omeprazole
South Africa
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Summary:To align predicted and measured CYP2C19 phenotype in a South African cohort. Genotyping of and was performed using PCR-RFLP, and an activity score (AS) system was used to predict phenotype. True phenotype was measured using plasma concentrations of omeprazole and its metabolite 5′-hydroxyomperazole. Partial genotype-phenotype discrepancies were reported, and an adapted AS system was developed, which showed a marked improvement in phenotype prediction. Results highlight the need for a more comprehensive genotyping approach to improve prediction of omeprazole metabolism. Evidence for the utility of a AS system is provided, for which the accuracy can be further improved by means of comprehensive genotyping and substrate-specific modification.
ISSN:1462-2416
1744-8042
DOI:10.2217/pgs.15.80