Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to delive...

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Published inNanomedicine (London, England) Vol. 10; no. 16; pp. 2513 - 2525
Main Authors Child, Hannah Winifred, Hernandez, Yulán, Conde, João, Mullin, Margaret, Baptista, Pedro, de la Fuente, Jesus Maria, Berry, Catherine Cecilia
Format Journal Article
LanguageEnglish
Published Future Medicine Ltd 01.08.2015
Subjects
apoptosis
c-myc
cell cycle
cell delivery
cell proliferation
gene knockdown
gold nanoparticles
knockdown
nanoparticles
off-target effects
proliferation
RNAi
siRNA
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Summary:: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. : Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. : The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. : The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm.15.95