Sodium ascorbate kills

Ascorbate can inhibit growth and even decrease viability of various microbial species including However the optimum conditions and the mechanism of action are unclear. shaken for 90 min in a buffered solution of ascorbate (90 mM) gave a 5-log reduction of cell viability, while there was no killing w...

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Published inFuture microbiology Vol. 11; no. 12; pp. 1535 - 1547
Main Authors Avci, Pinar, Freire, Fernanda, Banvolgyi, Andras, Mylonakis, Eleftherios, Wikonkal, Norbert M, Hamblin, Michael R
Format Journal Article
LanguageEnglish
Published Future Medicine Ltd 01.12.2016
Subjects
ascorbate
hydroxyl radicals
oxidative stress
vitamin C
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Summary:Ascorbate can inhibit growth and even decrease viability of various microbial species including However the optimum conditions and the mechanism of action are unclear. shaken for 90 min in a buffered solution of ascorbate (90 mM) gave a 5-log reduction of cell viability, while there was no killing without shaking, in growth media with different carbon sources or at 4°C. Killing was inhibited by the iron chelator 2,2′-bipyridyl. Hydroxyphenyl fluorescein probe showed the intracellular generation of hydroxyl radicals. Ascorbate-mediated killing of depends on oxygenation and metabolism, involves iron-catalyzed generation of hydroxyl radicals via Fenton reaction and depletion of intracellular NADH. Ascorbate could serve as a component of a topical antifungal therapy.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2016-0063