The use of enzyme-linked immunosorbent assay (ELISA) and complement fixation test to detect antibody response to Leptinotarsa interrogans serovar Hardjo in cattle

The enzime-linked immunosorbent assay (ELISA) and complement fixation test (CFT) were used to detect leptospira specific antibody response in cattle. ELISA antigen was developed from heated culture of a strain Wijnberg serovar Copenhageni and CFT antigen was developed from formolized culture of sero...

Full description

Saved in:
Bibliographic Details
Published inMikrobiologija (Moskva) Vol. 35; no. 1
Main Authors Kostovic, M. (Faculty of Veterinary Medicine, Belgrade (Yugoslavia)), Lako, B, Asanin, R
Format Journal Article
LanguageEnglish
Published 1998
Subjects
BOVIN
CATTLE
COMPLEMENT FIXATION TESTS
ELISA
GANADO BOVINO
IMMUNE RESPONSE
LEPTOSPIRA
PRUEBAS DE FIJACION DEL COMPLEMENTO
REACTION DE FIXATION DU COMPLEMENT
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
TEST ELISA
Online AccessGet more information

Cover

More Information
Summary:The enzime-linked immunosorbent assay (ELISA) and complement fixation test (CFT) were used to detect leptospira specific antibody response in cattle. ELISA antigen was developed from heated culture of a strain Wijnberg serovar Copenhageni and CFT antigen was developed from formolized culture of serovar Hardjo strain Hardjoprajitno. Microscopic agglutination test (MAT) served as reference. Low correlation of ELISA and high correlation of CFR with reference test were found. ELISA also showed lower specificity and sensitivity (88% and 91% respectively). Complement fixation test showed satisfying sensitivity (94%) and 100% specificity, which gave predictive value of a positive sample 100.00% and the predictive value of a negative test 99.76%.
Bibliography:2000000867
L73
ISSN:0581-1538