Immunochemical methods for fumonisins in corn

An enzyme-linked immunosorbent assay (ELISA) and an immunoaffinity column (IAC) cleanup procedure have been successfully applied to the determination of fumonisins in corn. The performance of the ELISA was evaluated by comparison to a reference high-performance liquid chromatographic (HPLC) method....

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Bibliographic Details
Main Author Trucksess, M.W. (U.S. Food and Drug Administration, Washington, DC.)
Format Book
LanguageEnglish
Published Washington, DC (USA) American Chemical Society 1996
Subjects
ELISA
HERBICIDAS
HERBICIDE
TEST ELISA
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Summary:An enzyme-linked immunosorbent assay (ELISA) and an immunoaffinity column (IAC) cleanup procedure have been successfully applied to the determination of fumonisins in corn. The performance of the ELISA was evaluated by comparison to a reference high-performance liquid chromatographic (HPLC) method. The IAC procedure was coupled with HPLC determination. The recoveries of fumonisins B1 from corn spiked at the 1, 2, and 4 micrograms/g levels were 73-106, 79-83, and 64-92% for the ELISA, IAC, and HPLC methods, respectively. The accuracy and precision of the methods compared favorably. In the comparative studies using naturally contaminated corn samples, the ELISA results were 2-100% higher than those determined by HPLC. The immunoaffinity procedure results were about 71% of the levels observed using HPLC
Bibliography:9738208
Q03
H20
ISBN:9780841234543
084123454X