A paramagnetic particle-based enzyme-linked immunosorbent assay for the quantitative determination of 3,5,6-trichloro-2-pyridinol in water

A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of 3,5,6-trichloro-2-pyridinol (TCP), the major biological and environmental degradation product of chlorpyrifos and triclopyr, was developed. Magnetic particles were used as the solid phase to attach monoclonal anti-TCP an...

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Bibliographic Details
Main Authors Itak, J.A. (Ohmicron Corporation, Newtown, PA.), Day, W.A, Montoya, A, Manclus, J.J, Phillips, A.M, Lindsay, D.A, Herzog, D.P
Format Book
LanguageEnglish
Published Washington, DC (USA) American Chemical Society 1997
Subjects
CHLORPYRIFOS
CLORPIRIFOS
ELISA
TEST ELISA
TRICLOPIR
TRICLOPYR
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Summary:A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of 3,5,6-trichloro-2-pyridinol (TCP), the major biological and environmental degradation product of chlorpyrifos and triclopyr, was developed. Magnetic particles were used as the solid phase to attach monoclonal anti-TCP antibodies. The ELISA has an estimated least detectable dose of 0.25 parts per billion (ppb; ng/mL) in water. Specificity studies indicate that the assay is specific for TCP and can distinguish it from the parent compounds as well as many other related and unrelated agricultural compounds. Results compare favorably with GC/MSD measurements (r = 0.959)
Bibliography:T01
9738160
ISBN:0841234876
9780841234871