Immunochemical approach for pesticide waste treatment monitoring of s-triazines

• Main Authors: Muldoon, M.T. (Food Animal Protection Research Laboratory, ARS, USDA, College Station, TX.), Nelson, J.O
• Format: Book
• Language: English
• Published: Washington, D.C. (USA) American Chemical Society 1995
• Subjects: GESTION DES DECHETS; MANEJO DE DESECHOS; PESTICIDE; PLAGUICIDAS; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS; TRIACINA; TRIAZINE
• ISBN: 0841231494; 9780841231498

Enzyme-linked immunosorbent assay (ELISA) was developed for the analysis of pesticide waste and rinsate and disposal monitoring. Three s-triazine-specific monoclonal antibodies that possessed different specificities were utilized to quantitate individual and total s-triazine analyses in mixtures. Results from the analysis of field samples were validated by HPLC. An immunoassay was developed for chlorodiamino-s-triazine (CAAT), an important degradation product of chloro-s-triazine herbicides. Antibody recognition of substituted s-triazines decreased as a function of increased amino side chain substitution. The assays were sensitive in the low micromolar range. An s-triazine herbicide class-specific ELISA was used in conjunction with an ELISA for CAAT for measuring s-triazine herbicide ozonation followed by microbiological treatment. The ELISAs were shown to be very accurate and precise for measuring the concentrations of both atrazine and CAAT. The information obtained by the two ELISAs could be used for on-site control of the two stage treatment process and should save time and expense in s-triazine disposal monitoring applications