Genotypic at the kappa-casein locus in cows using the polymerase chain reaction

In vitro amplification using the polymerase chain reaction was carried out to identify genotypes at the kappa-casein locus in cows. The amplified gene fragment contained a region in which nucleotide substitutions identify kappa-casein allelic variants and generate additional HindIII and TaqI sites....

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Published inSoviet genetics (USA) Vol. 27; no. 12
Main Authors Sulimova, G.E. (N.I. Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow), Shaikhaev, G.O, Berberov, E.M, Markaryan, A.Yu, Kandalova, L.G
Format Journal Article
LanguageEnglish
Russian
Published 01.06.1992
Subjects
BIOCHIMIE
BIOQUIMICA
BOVIN
CASEINA
CASEINE
GANADO BOVINO
GENE
GENES
GENOTIPOS
GENOTYPE
HETEROCIGOTOS
HETEROZYGOTE
LOCI
LOCUS
POLIMORFISMO
POLIMORFISMO GENETICO
POLYMORPHISME
POLYMORPHISME GENETIQUE
PRODUCTOS DEL CRUZAMIENTO
PRODUIT DE CROISEMENT
RACE
RAZAS
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Summary:In vitro amplification using the polymerase chain reaction was carried out to identify genotypes at the kappa-casein locus in cows. The amplified gene fragment contained a region in which nucleotide substitutions identify kappa-casein allelic variants and generate additional HindIII and TaqI sites. The specificity with which the kappa-casein gene fragment was amplified was confirmed by blot hybridization and restriction analysis. Digestion of the amplified fragment with PstI, HindIII, or TaqI, followed by electrophoresis in polyacrylamide or agarose gels, was used to identify the AA, AB and BB genotypes of animals at the kappa-casein locus. Allelic variants of the kappa-casein gene were identified in 32 crossbred animals (Jersey X variegated X Holstein-Friesian), using animals with known (18 animals) and unknown (14 animals) kappa-casein phenotypes. Results obtained by the PCR or restriction fragment length polymorphism methods agreed with those obtained by analysis of protein polymorphism in all cases. The kappa-casein B allele occurred at a high frequency (53.1 +/- 8.8%) in these animals, and heterozygosity was frequent, with the AB genotype occurring in 43.7% of animals. The use of PCR analysis for genotyping animals was shown to be efficient, and independent of lactation, as it was applicable to bulls and young cows: this is very important in agricultural practice. The advantages of this method in large-scale genotype testing in animals are discussed in relation to population and genetic breeding studies
Bibliography:L10
9303291
ISSN:0038-5409