Mutagenesis and repair processes in an artificially synchronized population of Crepis capillaris cells

A mitotic population of Crepis capillaris rootlet meristem cells, which was asynchronous after 10 h growth, was synchronized in the DNA synthesis phase using 5-aminouracil (5AU). The frequencies of chromosomal mutations induced by mitomycin C were studied in the naturally dividing and synchronized p...

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Bibliographic Details
Published inSoviet genetics (USA) Vol. 27; no. 6
Main Authors Dubinina, L.G. (Academy of Sciences of the USSR, Moscow), Kurashova, Z.I, Volkova, I.V
Format Journal Article
LanguageEnglish
Russian
Published 01.12.1991
Subjects
ADN
CHROMOSOME
COMPOSITAE
CROMOSOMAS
MITOSE
MITOSIS
MUTACION
MUTACION INDUCIDA
MUTATION
MUTATION PROVOQUEE
SINCRONIZACION
SYNCHRONISATION
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Summary:A mitotic population of Crepis capillaris rootlet meristem cells, which was asynchronous after 10 h growth, was synchronized in the DNA synthesis phase using 5-aminouracil (5AU). The frequencies of chromosomal mutations induced by mitomycin C were studied in the naturally dividing and synchronized populations, as were constitutive repair and the adaptive responses consisting of inducible repair, in cultures with cell cycles of different durations. Similar results were obtained for both types of population. Cell cycle block prevented repair activity which normally reduces the level of DNA damage, as shown by the identical levels of mutagenesis in the earlier mitoses in both populations despite the fact that early mitoses appeared 4 h later in the synchronized population
Bibliography:F30
9301111
ISSN:0038-5409