New firefly luciferase preparations for bioluminescence microanalysis

New methods for producing partially purified and immobilized luciferase have been devised on the basis of the notion of this enzyme as a lipoprotein. A highly active and stable soluble luciferase preparation has been obtained, making it possible to determine ATP concentrations down to 0.1 nM. The pr...

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Bibliographic Details
Published inSoviet biotechnology (USA) no. 3
Main Authors Dukhovich, A.F. (Lomonoson Moscow State University), Filippova, N.Yu, Ugarova, N.N
Format Journal Article
LanguageEnglish
Russian
Published 1990
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ADENOSINE TRIPHOSPHATE
ADENOSINTRIFOSFATO
COLEOPTERA
IMMOBILISATION
INMOVILIZACION
LECITHINE
LECITINAS
MEDICION
MEMBRANA
MEMBRANE
MESURE
OXIDORREDUCTASAS
OXYDOREDUCTASE
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
PURIFICACION
PURIFICATION
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Summary:New methods for producing partially purified and immobilized luciferase have been devised on the basis of the notion of this enzyme as a lipoprotein. A highly active and stable soluble luciferase preparation has been obtained, making it possible to determine ATP concentrations down to 0.1 nM. The present paper describes a method for immobilizing the enzyme on phosphatidylcholine-lipidized polymer membranes based on nitrocellulose and cellulose acetate. It has been shown that the immobilized luciferase preparations can be reused in bioluminescence microanalysis
Bibliography:9136124
L50
ISSN:0890-734X