Molecular characterization of a zygote wall protein: an extension-like molecule in Chlamydomonas reinhardtii

The green alga Chlamydomonas reinhardtii elaborates two biochemically and morphologically distinct cell walls during its life cycle: one surrounds the vegetative and gametic cell and the other encompasses the zygote. Hydroxyproline-rich glycoproteins (HRGPs) constitute a major component of both wall...

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Published inThe Plant cell Vol. 1; no. 9
Main Authors Woessner, J.P. (Washington University, St. Louis, MO), Goodennough, U.W
Format Journal Article
LanguageEnglish
Published 01.09.1989
Subjects
CHLOROPHYCEAE
CIGOTOS
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENES
GENETICA
GENETIQUE
GLICOPROTEINAS
GLYCOPROTEINE
NUCLEOTIDE
NUCLEOTIDOS
PARED CELULAR
PAROI CELLULAIRE
TECHNIQUE DE PREVISION
TECHNIQUE IMMUNOLOGIQUE
TECNICAS DE PREDICCION
TECNICAS INMUNOLOGICAS
ZYGOTE
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Summary:The green alga Chlamydomonas reinhardtii elaborates two biochemically and morphologically distinct cell walls during its life cycle: one surrounds the vegetative and gametic cell and the other encompasses the zygote. Hydroxyproline-rich glycoproteins (HRGPs) constitute a major component of both walls. We describe the isolation and characterization of a zygote-specific gene encoding a wall HRGP. The derived amino acid sequence of this algal HRGP is similar to those of higher plant extensins, rich in proline and serine residues and possessing repeating amino acid motifs, notably X(Pro)3 and (Ser-Pro)n. Antiserum against this zygote wall protein detected common epitopes in several other zygote polypeptides, at least one of which is also encoded by a zygote-specific gene. We conclude that there is one set of HRGP wall genes expressed only in zygotes and another set that is specific to vegetative and gametic cells
Bibliography:F60
F30
9123918
ISSN:1040-4651
1532-298X