RFLP analysis reveals the genealogical relation of novel Japanese persimmon âSaijoâ
âSaijoâ Japanese persimmon (Diospyros kaki Thumb.) is one of the most popular and commercially important fruit cultivars in Japan. In 2000, origin-unknown âSaijoâ-like cultivar was found at the Shimane farm and named as âM-3â. Although it has similar morphological and physiological chara...
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Published in | Scientia horticulturae Vol. 199; pp. 71 - 74 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
2016
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Subjects | |
Online Access | Get full text |
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Summary: | âSaijoâ Japanese persimmon (Diospyros kaki Thumb.) is one of the most popular and commercially important fruit cultivars in Japan. In 2000, origin-unknown âSaijoâ-like cultivar was found at the Shimane farm and named as âM-3â. Although it has similar morphological and physiological characteristics with âSaijoâ cultivars, its fruit size is apparently bigger than âSaijoâ making them an attractive candidate as novel valuable fruit for farm household. In this study, to elucidate the genealogical relationship of âM-3â, we conducted the restriction fragment length polymorphism (RFLP) analysis using genomic DNA of Japanese persimmon (âSaijoâ lines, pollinizers, and other cultivars that bloom a male flower) detected by cDNA probes involved in genes coding persimmon fruit ripening: 1-aminocyclopropane-1-carboxylate synthase 1 (ACCS-1), ACC oxidase 1 (ACCO-1), and polygalacturonase (PG-9). We demonstrated that âM-3â line shares several RFLPs specific to âSaijoâ lines, pollinizers, and other cultivars. We found that morphological features of fruit and leaf in âM-3â resembled that of âSaijoâ and pollinizer lines. These results strongly suggest that âM-3â is likely the seedlings crossbred by âSaijoâ and pollinizer cultivars. Our findings provide first insight of genealogical origins of valuable newly identified persimmon cultivar and an important suggestion to establish a new valuable âSaijoâ cultivar by cross breeding with pollinizer lines. |
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Bibliography: | http://dx.doi.org/10.1016/j.scienta.2015.12.011 |
ISSN: | 0304-4238 1879-1018 |