novel method to scale up fungal endophyte isolations

Studies involving fungal endophytes very often rely on surface-sterilisation of plant samples to enable endophyte isolations. However, surface-sterilisation can be very time-consuming, potentially limiting the number of samples processed. To overcome this limitation, a novel method was developed to...

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Bibliographic Details
Published inBiocontrol science and technology Vol. 25; no. 10; pp. 1208 - 1212
Main Authors Greenfield, Melinda, Pareja, Reynaldo, Ortiz, Viviana, Gómez-Jiménez, María I, Vega, Fernando E, Parsa, Soroush
Format Journal Article
LanguageEnglish
Published Taylor & Francis 2015
Subjects
biological control
containers
endophytes
fungi
leaves
plant tissues
roots
sampling
seedlings
stems
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Summary:Studies involving fungal endophytes very often rely on surface-sterilisation of plant samples to enable endophyte isolations. However, surface-sterilisation can be very time-consuming, potentially limiting the number of samples processed. To overcome this limitation, a novel method was developed to bulk surface-sterilise multiple plant tissue samples simultaneously and separately. The method relies on 24 perforated Falcon™ tubes, each containing a sample, sequentially transferred through a series of containers holding the sterilants. The samples that can be surface-sterilised using this method include roots, stems and leaves or entire seedlings. This method increased our throughput by a factor of 24 relative to conventional surface-sterilisation methods.
Bibliography:http://dx.doi.org/10.1080/09583157.2015.1033382
ISSN:1360-0478