epifluorescent attachment improves whole-plant digital photography of Arabidopsis thaliana expressing red-shifted green fluorescent protein

Filter cubes made with machine-vision dichroic filters and illuminated with a royal blue light emitting diode can be used to produce an epifluorescent digital camera attachment that improves whole organism green fluorescent protein (GFP) photography. Mean pixel intensity responds linearly to purifie...

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Bibliographic Details
Published inAoB plants Vol. 2012
Main Authors Baker, Stokes S, Vidican, Cleo B, Cameron, David S, Greib, Haittam G, Jarocki, Christine C, Setaputri, Andres W, Spicuzza, Christopher H, Burr, Aaron A, Waqas, Meriam A, Tolbert, Danzell A
Format Publication
LanguageEnglish
Published Oxford University Press 2012
Subjects
Arabidopsis thaliana
blue light
digital images
filters
green fluorescent protein
photography
titration
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Summary:Filter cubes made with machine-vision dichroic filters and illuminated with a royal blue light emitting diode can be used to produce an epifluorescent digital camera attachment that improves whole organism green fluorescent protein (GFP) photography. Mean pixel intensity responds linearly to purified GFP titration.
Bibliography:http://dx.doi.org/10.1093/aobpla/pls003