ELISA for guinea-pig IgG1: an alternative to the PCA test fo r relative allergenicity

An ELISA method to replace the PCA (passive cutaneous anaphylaxis) test has been developed for use in the evaluation of relative allergenicity in guinea-pigs (Dunkin-Hartley). The method detects antigen-specific IgG1 antibodies formed in the guinea-pig during sensitisation, and correlates well with...

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Bibliographic Details
Published inAlternatives to laboratory animals Vol. 19; no. 1; pp. 8 - 14
Main Author Prento, A
Format Journal Article
LanguageEnglish
Published 1991
Subjects
animal use alternatives
enzyme-linked immunosorbent assay
hypersensitivity
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Summary:An ELISA method to replace the PCA (passive cutaneous anaphylaxis) test has been developed for use in the evaluation of relative allergenicity in guinea-pigs (Dunkin-Hartley). The method detects antigen-specific IgG1 antibodies formed in the guinea-pig during sensitisation, and correlates well with the corresponding PCA test. The relative importance of IgG1 and IgE for relative allergenicity in the Dunkin-Hartley guinea-pig strain is discussed.
ISSN:0261-1929