high mobility group of nuclear proteins as biomarkers of age an d caloric restriction in rats

The quantitative levels and phosphorylation states of the high mobility group (HMG) of proteins were investigated in bone marrow, brain, heart, kidney, liver, pancreas, spleen, testis and thymus of three groups of male Fischer 344 rats. Two groups of rats, young ad libitum (Y/AL - 1 1/2 mo.) and old...

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Published inBiochemistry international Vol. 26; no. 5; pp. 953 - 961
Main Authors Pipkin, J.L, Hinson, W.G, Lyn-Cook, L.E, Feuers, R.J, Burns, E.R, Leakey, J.E.A, Hart, R.W, Casciano, D.A
Format Journal Article
LanguageEnglish
Published 1992
Subjects
age
energy deficiencies
markers
nucleoproteins
phosphorylation
rats
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Summary:The quantitative levels and phosphorylation states of the high mobility group (HMG) of proteins were investigated in bone marrow, brain, heart, kidney, liver, pancreas, spleen, testis and thymus of three groups of male Fischer 344 rats. Two groups of rats, young ad libitum (Y/AL - 1 1/2 mo.) and old ad libitum (O/AL - 28 mo.), had free access to at chow, and a third group of old rats were maintained on a caloric restricted intake (O/CR - 28 mo.). The quantities of HMGs 1, 2, 14 and 17 were significantly reduced in O/AL rats compared with Y/AL rats in all tissues examined, and in many cases, the amount of HMGs of O/CR rats were increased by varying degrees from O/AL animals. In G2-phase nuclei of bone marrow, spleen and testis, phosphorylation of HMG proteins was reduced significantly in O/AL rats, but was enhanced in O/CR animals (especially HMG14). These levels of HMGs in O/CR animals, altered by age and diet dependent actors, reflect a condition which is more reminiscent of Y/AL than O/AL animals.
ISSN:0158-5231