Characterisation by 1H-n.m.r. spectroscopy of oligosaccharides, derived from arabinoxylans of white endosperm of wheat, that contain the elements linked to 4 [alpha-L-Araf-(1 linked to 3)]-beta-D-Xylp-(1 linked to or linked to 4)[alpha-L-Araf-(1 linked to 2)][alpha-L-Araf-(1 linked to 3)]-beta-D-Xylp-1 linked to

The structure of penta- to hepta-saccharides, generated by digestion of purified wheat-endosperm arabinoxylan with endo-(1 linked to 4)-beta-D-xylanase and isolated by gel-permeation chromatography on Bio-Gel P-6 followed by high-performance anion-exchange chromatography with pulsed amperometric det...

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Bibliographic Details
Published inCarbohydrate research Vol. 221; pp. 63 - 81
Main Authors Hoffmann, R.A, Leeflang, B.R, Barse, M.M.J. de, Kamerling, J.P, Vliegenthart, J.F.G
Format Journal Article
LanguageEnglish
Published 1991
Subjects
arabinose
carbohydrate structure
chemical analysis
endosperm
hydrolysis
methylation
monosaccharide sequences
monosaccharides
oligosaccharides
purification
spectral analysis
Triticum aestivum
xylan
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Summary:The structure of penta- to hepta-saccharides, generated by digestion of purified wheat-endosperm arabinoxylan with endo-(1 linked to 4)-beta-D-xylanase and isolated by gel-permeation chromatography on Bio-Gel P-6 followed by high-performance anion-exchange chromatography with pulsed amperometric detection, was established using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The oligosaccharides had a core of (1 linked to 4)-linked beta-D-xylopyranosyl residues 3- or 2,3-substituted with single alpha-L-arabinofuranosyl groups, and gave 1H-n.m.r. spectra typical for each type.
ISSN:0008-6215
1873-426X