Tomato extension and extension-like cDNAs: structure and expression in response to wounding

• Published in: Plant molecular biology Vol. 16; no. 4; pp. 547 - 565
• Main Authors: Showalter, A.M, Zhou, J, Rumeau, D, Worst, S.G, Varner, J.E
• Format: Journal Article
• Language: English
• Published: 1991
• Subjects: amino acid sequences; cloning; ddbj/x55681; ddbj/x55682; ddbj/x55683; ddbj/x55684; ddbj/x55685; ddbj/x55686; ddbj/x55687; ddbj/x55688; ddbj/x55689; ddbj/x55690; ddbj/x55691; ddbj/x55692; ddbj/x55693; ddbj/x55694; ddbj/x55695; ddbj/x55696; DNA; embl/x55681; embl/x55682; embl/x55683; embl/x55684; embl/x55685; embl/x55686; embl/x55687; embl/x55688; embl/x55689; embl/x55690; embl/x55691; embl/x55692; embl/x55693; embl/x55694; embl/x55695; embl/x55696; genbank/x55681; genbank/x55682; genbank/x55683; genbank/x55684; genbank/x55685; genbank/x55686; genbank/x55687; genbank/x55688; genbank/x55689; genbank/x55690; genbank/x55691; genbank/x55692; genbank/x55693; genbank/x55694; genbank/x55695; genbank/x55696; gene expression; genes; glycoproteins; hydroxyproline; hydroxyproline-rich glycoproteins; injuries; messenger RNA; molecular sequence data; nucleotide sequences; restriction mapping; RNA; Solanum lycopersicum var. lycopersicum
• ISSN: 0167-4412; 1573-5028

Two tomato cDNA libraries were synthesized from poly(A)+ RNAs isolated from unwounded and wounded tomato stems. These cDNA libraries were packaged inlambda gt(10) and screened by in situ plaque hybridization with a tomato extensin gene clone (pTom 5.10).Several cDNA clones were identified and isolated from both libraries in this manner and subjected torestriction enzyme digestion, Southern gel blot hybridization, RNA gel blot hybridization, and DNA sequence analyses. From these analyses, the various cDNA clones were found to fall into one of five distinctclasses (classes I-V). Class I clones hybridized to a 4.0 kb mRNA which accumulated markedly afterwounding and encoded an extensin characterized largely by Ser-(Pro)4-Ser-Pro-Ser-(Pro)4-(Tyr)3-Lys repeats. Class II clones hybridized to a 2.6 kb mRNA which showed no accumulation following wounding andencoded an extensin containing Ser-(Pro)4-Ser-Pro-Ser-(Pro)4-Thr-(Tyr)1-3-Ser repeats. Class IIIclones hybridized to a 0.6 kb mRNA which greatly accumulated in response to wounding andencoded a glycine-rich protein (GRP) with (GlY)2-6-Tyr-Pro and(Gly)2-6-Arg repeats. Class IV clonescontained both class I and class Ill DNA sequences and consequently hybridized to both the 4.0 kb andthe 0.6 kb wound-accumulating mRNAS; these clones encoded a portion of a GRP sequence on oneDNA strand and encoded a portion of an extensin sequence on the other DNA strand. Class V clones hybridized to a 2.3 kb mRNA which decreased following wounding and encoded a GRP sequencecharacterized by (Gly)2-5-Arg repeats.