Predictive and prognostic markers of breast cancer

A multitude of prognostic and predictive multiparameter algorithms based on the analysis of mRNA have been published in recent years. Many of the algorithms require fresh or fresh frozen tissue as a source of the mRNA. However, practical considerations suggest formalin-fixed paraffin-embedded tissue...

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Bibliographic Details
Published inDer Pathologe Vol. 29; no. 2; pp. 181 - 183
Main Authors Petry, C, Gehrmann, M, von Törne, C, Weber, K, Stropp, U, Hennig, G
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg : Springer-Verlag 2008
Subjects
DNA
Extraction method
messenger RNA
Node-negative breast cancer
Prognosis test
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Summary:A multitude of prognostic and predictive multiparameter algorithms based on the analysis of mRNA have been published in recent years. Many of the algorithms require fresh or fresh frozen tissue as a source of the mRNA. However, practical considerations suggest formalin-fixed paraffin-embedded tissue (FFPE tissue) to be a more suitable starting material for routine diagnostic applications. Therefore, Siemens Healthcare Diagnostics is developing a fully automated method to extract mRNA and DNA from FFPE tissue for use in pathology laboratories. Initially, the method will be used as part of a prognosis assay to predict the likelihood of distant metastasis and death for node-negative breast cancer patients.
Bibliography:http://dx.doi.org/10.1007/s00292-008-1081-4
ISSN:0172-8113
1432-1963