Charcterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase

Xyloglucan endo-transglycosylase (XET) catalyses the cleavage and concomitant transfer of one xylo-glucan molecule to another. It is thought to be an important component of cell wall metabolism, particularly in expanding tissue and ripening fruits. The recently reported cloning of a cDNA encoding a...

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Bibliographic Details
Published inPlant molecular biology Vol. 28; no. 3; pp. 391 - 403
Main Authors Arrowsmith, D.A, De Silva, J
Format Journal Article
LanguageEnglish
Published 1995
Subjects
amino acid sequences
complementary DNA
enzyme activity
fruits
genbank/x82683
genbank/x82684
genbank/x82685
gene expression
messenger RNA
molecular sequence data
multigene family
nucleotide sequences
ripening
Solanum lycopersicum var. lycopersicum
transferases
xyloglucans
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Summary:Xyloglucan endo-transglycosylase (XET) catalyses the cleavage and concomitant transfer of one xylo-glucan molecule to another. It is thought to be an important component of cell wall metabolism, particularly in expanding tissue and ripening fruits. The recently reported cloning of a cDNA encoding a seed-expressed XET from nasturtium has enabled two XET-encoding cDNAs to be isolated from tomato fruit (breaker stage) cDNA library. Their deduced amino acid sequences exhibit ca. 40% identity to nasturtium XET. One of the tomato cDNA clones (tXET-B2) was over-expressed in Escherichia coli; following purification and refolding, the recombinant protein was shown to have XET activity with no detectable hydrolytic activity. Southern hybridisation analysis suggests that these clones are members of a small multi-gene family encoding tomato XET. Ribonuclease protection assays show that transcripts protected by one of the clones (tXET-B1) are most abundant in pink fruit pericarp and were also detected in stems.
ISSN:0167-4412
1573-5028