In vitro culture of Cucumis sativus L. XVII. Plants from protoplasts through direct somatic embryogenesis

A procedure is described for the isolation and culture of protoplasts from embryogenic callus (gel-like callus GLC) and embryogenic suspension cultures (ESC) of Cucumis sativus c.v. Borszczagowski. Maximal protoplast yields from GLC and ESC were 5 X 10(6) and 1 X 10(7) protoplasts/g tissue respectiv...

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Published inPlant cell, tissue and organ culture Vol. 41; no. 3; pp. 259 - 266
Main Authors Burza, W, Malepszy, S
Format Journal Article
LanguageEnglish
Published 1995
Subjects
callus
cell suspension culture
Cucumis sativus
culture media
explants
in vitro culture
leaves
protoplasts
regenerative ability
somatic embryogenesis
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Summary:A procedure is described for the isolation and culture of protoplasts from embryogenic callus (gel-like callus GLC) and embryogenic suspension cultures (ESC) of Cucumis sativus c.v. Borszczagowski. Maximal protoplast yields from GLC and ESC were 5 X 10(6) and 1 X 10(7) protoplasts/g tissue respectively. They were obtained following 14-16 h digestion with 1.2% Cellulase Onozuka R-10, 1.2% Macerozyme R-10 and 0.3% Driselase. At a plating density of 2 X 10(5)/ml, first divisions occurred in 4-5 days and 7-8 days in ESC- and GLC-derived protoplasts respectively. The highest percentage of direct embryogenesis (over 80%) was observed with ESC. It was possible to obtain approximately 5000 embryo structures/g tissue. Some embryos converted into plants after 6 weeks, but most of them after 2 months of culture. ESC-derived plants, when transferred into the glasshouse, bloomed normally, and set seeds.
ISSN:0167-6857
1573-5044