Rapid micropropagation of Woodfordia fruiticosa (L.) Kurz (Lythraceae), a rare medicinal plant

A rapid propagation method comprising initiation of in vitro shoot tip culture from field-grown flowering plants and reculture of the nodal segments of regenerated shoots in Schenk and Hildebrandt (1972) medium was developed for Woodfordia fruticosa (L.) Kurz., a rare medicinal shrub. A medium suppl...

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Bibliographic Details
Published inPlant cell reports Vol. 14; no. 1; pp. 55 - 58
Main Authors Krishnan, P.N, Seeni, S
Format Journal Article
LanguageEnglish
Published 1994
Subjects
benzyladenine
culture media
explants
growth
indole acetic acid
micropropagation
naphthaleneacetic acid
rapid methods
regenerative ability
shoot tip culture
shoots
stems
tissue culture
Woodfordia fruticosa
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Summary:A rapid propagation method comprising initiation of in vitro shoot tip culture from field-grown flowering plants and reculture of the nodal segments of regenerated shoots in Schenk and Hildebrandt (1972) medium was developed for Woodfordia fruticosa (L.) Kurz., a rare medicinal shrub. A medium supplement of 6-benzylaminopurine (0.2 mg.1(-1)) induced high frequency (88%) development of axillary shoot buds (3.2) in 4-5 weeks. Subculture of the explants with multiple new shoots in fresh medium for 30 days yielded an even larger number (9.7) of shoots. Highest multiplication (26-35 shoots) was recorded when using culture initiation media with 0.5 mg.1(-1) each of BAP and NAA followed by subculture in 0.2 mg.1(-1) BAP. The shoot multiplication rate was further accelerated by reculturing 0.4-0.6 cm nodal segments of regenerated shoots in media with 1.0 mg.1(-1) BAP. Shoot cuttings (3.5-7.0 cm) were rooted in 0.2 mg.1(-1) IAA. Regenerated plants displayed uniform morphological, growth and flowering characteristics.
ISSN:0721-7714
1432-203X