Anther culture n Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration

• Published in: Plant cell reports Vol. 13; no. 3/4; pp. 222 - 226
• Main Authors: Thengane, S.R, Joshi, M.S, Khuspe, S.S, Mascarenhas, A.F
• Format: Journal Article
• Language: English
• Published: 1994
• Subjects: anther culture; culture media; embryo (plant); genetic variation; genotype; Helianthus annuus; induction; plant anatomy; plant growth substances; plant morphology; regenerative ability; sucrose
• ISSN: 0721-7714; 1432-203X

Production of microspore- derived embryos from Cultured anthers is now a well established technique for the isolation of homozygous lines in many crop plants. We describe here a culture method for embryo induction and plant regeneration from anthers of four sunflower genotypes. For preliminary experiments, anthers of uninucleate microspores were cultured on four types of basal media viz., Murashige and Skoog's MS, Gamborg's B5, Nitsch and Nitsch, and White's W, supplemented with 1.0 mg/l 2,4 dichlorophenoxy acetic acid and 0.5 mg/l 6-benzylaminopurine and 40 g/l sucrose. MS basal medium, being more responsive for embryo induction, was used for further experimentation. To optimise the culture requirement MS basal medium was supplemented with 0.2-2.0 mg/l 2,4 dichlorophenoxy acetic acid and 0.5 and 1.0 mg/l 6-benzylaminopurine. The effect of cold pretreatment e and sucrose concentration were tested c efficiency. Genotype had a significant e ct on the capacity of embryo induction. Addition of silver nitrate (2.5 mg/l), an ethylene inhibitor, stimulated embryo germination. Plantlets were obtained (10-15%) from embryos of only one genotype.