Adhesion properties, fimbrial expression and PCR detection of adhesion-related genes of avian Escherichia coli strains

Forty-nine avian pathogenic Escherichia coli (APEC) strains obtained from chickens suffering from septicemia (24), swollen head syndrome (14) and omphalitis (11), isolated from individuals in different regions of Brazil and from different outbreaks, were studied for their adhesion to trachea epithel...

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Published inVeterinary microbiology Vol. 106; pp. 275 - 285
Main Authors Campos, T.A. de, Stehling, E.G, Ferreira, A, Castro, A.F.P. de, Brocchi, M, Silveira, W.D. da
Format Journal Article
LanguageChinese
Published 2005
Subjects
adhesins
bacteremia
bacterial adhesion
chickens
commensalism
disease detection
disease outbreaks
Escherichia coli
Escherichia infections
fimbriae
gene expression
hemagglutination tests
hemagglutinins
host-pathogen relationships
microbial genetics
molecular sequence data
nucleotide sequences
omphalitis
pathogenicity
polymerase chain reaction
poultry diseases
strain differences
swollen head syndrome
trachea (vertebrates)
Brazil
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Summary:Forty-nine avian pathogenic Escherichia coli (APEC) strains obtained from chickens suffering from septicemia (24), swollen head syndrome (14) and omphalitis (11), isolated from individuals in different regions of Brazil and from different outbreaks, were studied for their adhesion to trachea epithelial cells, fimbrial expression and hemagglutination capacity to different erythrocyte types. These results were compared with their content of fimbriae-related genes as detected by polymerase chain reaction (PCR) using specific pair of primers. The aim of these assays was to determine the importance of expression of adhesins in the pathogenic strains and to evaluate the presence of adhesin genes either previously described or not yet recognized for APEC strain. Thirty commensal strains isolated from poultry showing no signs of any of the above diseases were used to compare the results with the pathogenic isolates. The PCR assay demonstrated that septicaemic and swollen head syndrome strains had the highest number o
ISSN:0378-1135
1873-2542