Heterogeneous distribution of Escherichia coli O157 within naturally infected bovine faecal pats

Escherichia coli O157 is an important human pathogen for which cattle are considered a reservoir. This paper describes and models the variation in counts of E. coli O157 that exists within individual bovine faecal pats. The presence and concentration of E. coli O157 in faecal samples was determined...

Full description

Saved in:
Bibliographic Details
Published inFEMS microbiology letters Vol. 244; no. 2; pp. 291 - 296
Main Authors Robinson, S.E, Brown, P.E, Wright, E.J, Bennett, M, Hart, C.A, French, N.P
Format Journal Article
LanguageEnglish
Published 2005
Subjects
animal pathogenic bacteria
cattle
disease reservoirs
feces
humans
intestinal microorganisms
microbial heterogeneity
Online AccessGet full text

Cover

More Information
Summary:Escherichia coli O157 is an important human pathogen for which cattle are considered a reservoir. This paper describes and models the variation in counts of E. coli O157 that exists within individual bovine faecal pats. The presence and concentration of E. coli O157 in faecal samples was determined using a combination of direct spiral plating followed by a more sensitive isolation procedure. The data were modelled using multilevel random effect models, in which the random effects were allowed to be correlated to allow for the fact that pooled and individual samples come from the same pat. Up to a two log difference in the concentration of E. coli O157 was demonstrated in samples from different areas within a faecal pat. Pooling of individual samples from throughout the faecal pat and processing it as one composite sample allows this heterogeneity to be overcome.
ISSN:0378-1097
1574-6968