Apple polyphenol oxidase inhibitor(s) from common housefly (Musca domestica L.)
A new natural apple polyphenol oxidase (PPO) inhibitor(s) from housefly (Musca domestica L.) was discovered. Crude inhibitor (s) isolated by buffer extraction, heat treatment, and dialysis from housefly pupae inhibited the activity of apple PPO up to 90% at pH values above 5.0. Inhibition was strict...
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Published in | Journal of food science: an official publication of the Institute of Food Technologists Vol. 68; no. 6; pp. 1942 - 1947 |
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Main Authors | , , , |
Format | Publication |
Language | English |
Published |
2003
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Subjects | |
Online Access | Get more information |
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Summary: | A new natural apple polyphenol oxidase (PPO) inhibitor(s) from housefly (Musca domestica L.) was discovered. Crude inhibitor (s) isolated by buffer extraction, heat treatment, and dialysis from housefly pupae inhibited the activity of apple PPO up to 90% at pH values above 5.0. Inhibition was strictly pH-dependent. The inhibitor(s) was further characterized by employing heat, freezing and thawing, irradiation, pH adjustment, and ultrafiltration studies. The potential PPO inhibitor(s) was stable to heating at 100 °C for 1 h, repeated freezing and thawing, and irradiation. The inhibitor(s) was most stable at pH around 5.0 and least stable at alkaline pH. The PPO inhibition profile of housefly during metamorphosis also was evaluated. |
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Bibliography: | http://hdl.handle.net/10113/3754 |