Screening of Haematobia irritans (Diptera: Muscidae) populations for pyrethroid resistance-associated sodium channel gene mutations by using a polymerase chain reaction assay

A polymerase chain reaction (PCR)-based rapid screening procedure was developed to test individual horn flies, Haematobia irritans irritans (L.), for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with pyrethroid resistance. By a syste...

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Bibliographic Details
Published inJournal of medical entomology Vol. 35; no. 5
Main Authors Guerrero, F.D. (Knipling-Bushland U.S. Livestock Insects Research Laborotory, USDA-ARS, Kerrville, TX.), Kunz, S.E, Kammlah, D
Format Journal Article
LanguageEnglish
Published 01.09.1998
Subjects
ALLELES
CIHALOTRINA
CONTROL DE INSECTOS
CYHALOTHRIN
CYHALOTHRINE
ENSAYO
GENE
GENES
GENETIC RESISTANCE
HAEMATOBIA IRRITANS
HAEMATOBIA IRRITANS IRRITANS
INSECT CONTROL
INSECTICIDAS
INSECTICIDE
INSECTICIDES
ION
ION TRANSPORT
IONES
IONS
KDR MUTATIONS
LUTTE ANTIINSECTE
MUTACION
MUTATION
NUCLEOTIDE SEQUENCE
PCR
RESISTANCE ALLELES
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTANCE GENETIQUE
RESISTANCE TO CHEMICALS
RESISTENCIA A PRODUCTOS QUIMICOS
RESISTENCIA GENETICA
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
SODIO
SODIUM
TESTAGE
TESTING
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Summary:A polymerase chain reaction (PCR)-based rapid screening procedure was developed to test individual horn flies, Haematobia irritans irritans (L.), for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with pyrethroid resistance. By a systematic optimization of reaction conditions and judicious choice of PCR primers differing in DNA sequence by a single nucleotide, we identified pyrethroid-susceptible or resistant sodium channel alleles in individual flies. Laboratory and field populations were examined by both the PCR assay and conventional filter paper bioassays with the pyrethroid cyhalothrin to verify that populations containing greater proportions of individuals with the resistant sodium channel allele DNA sequence also had higher bioassay LC50 values. The PCB assay for resistance alleles gave definitive information on the genotype of an individual fly and detected the presence of heterozygous individuals that might serve as reservoirs of resistance genes in field populations
Bibliography:L72
1999003513
ISSN:0022-2585
1938-2928