Response of animals to vaccination against anthrax as monitored by ELISA

Study is being undertaken to determine the antibody response as measured by ELISA of large and small ruminants to vaccination with a commercial Bacillus anthracis spore vaccine. Groups of sheep and cattle were vaccinated with the recommended dose of vaccine. Blood samples were collected on a regular...

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Bibliographic Details
Main Authors Hardjoutomo, S, Patten, B.E, Barkah, K. (Research Inst. for Veterinary Science, Bogor (Indonesia))
Format Conference Proceeding
LanguageEnglish
Published Bangkok (Thailand) 1990
Subjects
BACILLUS ANTHRACIS
BOVIN
CARBUNCLO BACTERIANO
CHARBON BACTERIDIEN
ELISA
GANADO BOVINO
IMMUNOLOGIE
INMUNOLOGIA
OVIN
OVINOS
TEST ELISA
VACCINATION
VACUNACION
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Summary:Study is being undertaken to determine the antibody response as measured by ELISA of large and small ruminants to vaccination with a commercial Bacillus anthracis spore vaccine. Groups of sheep and cattle were vaccinated with the recommended dose of vaccine. Blood samples were collected on a regular basis over the following year. An ELISA test was established using a washed cell preparation from a solid agar culture of B. anthracis cell as the ELISA antigen. Polyacrylamide gel electrophoresis of the antigen showed the presence of a number of proteins including toxin components. All groups of animals responded to the vaccination however the antibody response of the sheep was more marked than the response of the cattle to the vaccination. Several cattle that received multiples doses of vaccine still produced a poor antibody response. Further work is underway to purify the ELISA antigen and to try and correlate the antibody response of the animals with protection to infection.
Bibliography:9321823
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