Prevalence, risk factor and genotyping of Cryptosporidium spp. from faces of dairy cow in Saraburi, Kanchanaburi and Nakornpathom provinces

• Main Authors: Tawin Inpankaew(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Parasitology) E-mail:fvettwi@ku.ac.th, Pipat Arunvipas(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Farm Resources and Production Medicine), Nongnuch Pinyopanuwat(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Parasitology), Wissanuwat Chimnoi(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Parasitology), Sathaporn Jittapalapong(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Parasitology)
• Format: Conference Proceeding
• Language: English
• Published: Bangkok (Thailand) 2009
• Subjects: Cryptosporidium; Dairy cattle; Dairy cows; Faeces; Genotypes; Infection; Morbidity; PCR-RFLP; Protozoa; Risk factors; Thailand

Aims of this study were to determine the prevalence, risk factors and genotype of Cryptosporidium spp. in dairy cows raised in the western part of Thailand including, Saraburi, Kanchanaburi and Nakornpatum. A total of 400 fecal samples were randomly collected and examined for Cryptosporidium spp. by the acid fast stain technique and polymerese chain reaction restriction fragment length polymorphism (PCR-RFLP). The prevalence of Cryptosporidium infection in dairy cows was 0.5 percent (2/400) by acid-fast staining and 1.3 percent (5/400) by PCR-RFLP. Age was the only significant risk factor for infection in dairy cows (p LT 0.05). All PCR positive samples were C.parvum (bovine genotype). The result indicated a potential risk of cryptosporidiosis transmission to human population, particularly in workers closely contact to cows.