Proteomic and characterization of alpha-amylase inhibitor from mungbean (Vigna radiate)

Callosobruchus maculatus causes damaging of storage mungbean seeds, leading to economical losses. For this reason the use of alpha-amylase inhibitors to interfere with the pest digestion process has been an interesting alternative as a biocontrolling agent. The studies of mutant mungbean by gramma r...

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Main Authors Anussorn Wisessing(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry), Arunee Engkagul(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry), Arunee Wongpiyasatid(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Applied Radiation and Isotopes), Kiattawee Chuwongkomon(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry) E-mail:fsciktc@ku.ac.th
Format Conference Proceeding
LanguageEnglish
Published Bangkok (Thailand) 2008
Subjects
Alpha amylase
Alpha-amylase inhibitor
Callosobruchus maculatus
Efficiency
Enzyme activity
Growth control
Insect control
Mung beans
Mungbean seeds
Pest insects
Quality
Seed damaging insects
Seeds
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Summary:Callosobruchus maculatus causes damaging of storage mungbean seeds, leading to economical losses. For this reason the use of alpha-amylase inhibitors to interfere with the pest digestion process has been an interesting alternative as a biocontrolling agent. The studies of mutant mungbean by gramma radiation, M5-16, found that the alpha-amylase inhibitor from the mutant lines was more inhibitory effective to C. maculates alpha-amylase than its wild type, KPS1 (Engkagul et al., 2004). To investigate this mechanism, both proteomics and biochemical characterization were employed. The alpha-amylase inhibitor from the mungbean seed, M5-16, was purified in two step procedures involving ammonium sulfate precipitation and gel filtration chromatography using sephadex G-100. This alpha-amylase inhibitor exits as a monomer and has a molecular weight of 27 kDa. Moreover, It is very interesting that the alpha-amylase inhibitor from the mungbean seed can inhibit C. maculates alpha-amylase but can not inhibit human salivary alpha-amylase. 2D gel electrophoresis suggested that gamma-induced mutant line decrease expression of alpha-amylase inhibitor and other protein comparing with wild type.
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