Porcine blood as control materials for animal blood cell analysis

Control material is an important component to verify the quality of animal blood cell analysis. In this study, 2 in-house control materials, denoted as normal and abnormal levels, were prepared from the porcine blood by fixing cells with 0.4 percent glutaraldehyde and 2.5 percent formaldehyde and ke...

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Main Authors Phantip Vattanaviboon(Mahidol University, Bangkok (Thailand). Faculty of Medical Technology. Department of Clinical Microscopy) E-mail:mtpvt@mahidol.ac.th, Sophon Sirisali(Pramongkutklao College of Medicine, Bangkok (Thailand). Department of Microbiology), Kulnaree Sirisali(Mahidol University, Bangkok (Thailand). Faculty of Medical Technology. Department of Clinical Chemistry), Sudarat Manochiopinij(Mahidol University, Bangkok (Thailand). Faculty of Medical Technology. Department of Clinical Chemistry), Pairoj Leelahakul(Mahidol University, Bangkok (Thailand). Faculty of Medical Technology. Department of Clinical Chemistry), Wijit Wonglumsom(Mahidol University, Bangkok (Thailand). Faculty of Medical Technology. Department of Microbiology)
Format Conference Proceeding
LanguageEnglish
Published Bangkok (Thailand) 2008
Subjects
Analytical methods
Blood
Blood cells
Blood sampling
Porcine blood
Quality controls
Swine
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Summary:Control material is an important component to verify the quality of animal blood cell analysis. In this study, 2 in-house control materials, denoted as normal and abnormal levels, were prepared from the porcine blood by fixing cells with 0.4 percent glutaraldehyde and 2.5 percent formaldehyde and kept at 4 deg C. The results obtained from weekly analyzed controls by automated cell analyzer showed that all 10 hematological parameters (WBC, RBC, HB, HCT, MCV, MCH, MCHC, RDW, PLT, MPV) of normal control was stable for a period of 5 weeks without any significant change from the original (p GT 0.05). But abnormal control demonstrated differently, the MPV value was significantly increased while MCHC value was significantly decreased (p LT 0.05). The other parameters were stable as found in the normal one. The hematological parameters of porcine blood are similar to those of various animals. The advantageous view is that a large amount of porcine blood could be conveniently collected from a slaughterhouse, therefore, it would be an unlimited sample source for preparing in-house control materials for animal blood cell analysis.
Bibliography:L50