Effect of herbal-extracted lotion and organic acid against Malassezia pachydermatitis in vitro

Fungicidal activity of herbal-extracted lotions, KU Natural Miticide, KU herbfungusmite, KU herbmalacide, KU Thong-phan-chang, KU Ta-Kai-Hom, KU Ta-Kai-Kaeng, Betel lotions, and 3 percent organic acids (acetic, citric, lactic, malic, oxalic, succinic and tartaric acids were in vitro tested against t...

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Main Authors Narong Chungsamarnyart(Kasetsart University. Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Faculty of Veterinary Medicine) E-mail:fvetnrc@ku.ac.th, Srisamai Viriyarampa(Kasetsart University. Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Faculty of Veterinary Medicine), Suwanna Thipayarak(Kasetsart University. Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Faculty of Veterinary Medicine), Julee Pankumnoed(Kasetsart University. Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Faculty of Veterinary Medicine), Thanasorn Thipayarak(Kasetsart University. Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Faculty of Veterinary Medicine)
Format Conference Proceeding
LanguageEnglish
Published Bangkok (Thailand) 2008
Subjects
Dogs
Eczema
Herbaceous plants
Herbal-extracted
In vitro
Malassezia pachydermatitis
Organic acids
Skin
Skin diseases
Yeasts
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Summary:Fungicidal activity of herbal-extracted lotions, KU Natural Miticide, KU herbfungusmite, KU herbmalacide, KU Thong-phan-chang, KU Ta-Kai-Hom, KU Ta-Kai-Kaeng, Betel lotions, and 3 percent organic acids (acetic, citric, lactic, malic, oxalic, succinic and tartaric acids were in vitro tested against the Malassezia pachydermatitis yeast. Ten tubes of yeast growth on potato dextrose agar (PDA) were soaked with each lotion and shook for 30 seconds, poured the lotion out and left it dry for 90 min. The control was sterile water. The subculture of treated yeast were done twice at after dry and subsequently after incubation at 37 deg C for 2 weeks. The subculture yeast from the control tubes grew normally in both two subcultures. While the both two subculture from the KU Natural Miticide, KU herbmalacide, KU Thong-phan-chang, KU Ta-Kai-Hom and KU Ta-Kai-Kaeng lotion-treated tubes did not grow, except in nine tubes of the first subculture from the KU herbfungusmite and acetic acid lotion-treated tubes were no yeast growth. These effective lotions were two times diluted by Sabouraud dextrose broth containing yeast (2*10**(5) colonies/ml), shook for 5 second, left contact for 5 min and poured on PDA. All of them showed no yeast growth, exception from acetic acid. This indicated that these KU herbal-extracted lotions might be fungicide for M. pachydermatitis.
Bibliography:L72