Conservation of White Lamphun cattle by cloning technique

• Main Authors: Kwanrudee Keawmungkun(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology) E-mail:k_keawmungkun@yahoo.com, Kanokwan Srirattana(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Sumeth Imsoonthornruksa(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Chuti Laowtammathron(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Chanchao Lorthongpanich(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Nucharin Sripunya(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Wanwisa Phewsoi(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Anawat Sangmalee(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Retvin Davahudee(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Rung Juntaboon(Chang Rung Farm, Chiang Mai (Thailand)), Mariena Ketudat-Cairns(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Rangsun Parnpai(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology)
• Format: Conference Proceeding
• Language: Thai
• Published: Bangkok (Thailand) 2008
• Subjects: Cattle; Cloning; Ears; Embryo transfer; Fibroblasts; Growth rate; Oestrous cycle; Parthenogentic activation; Pregnancy; Varieties; White Lamphun cattle

White Lamphun cattle is a native breed of the Northern part of Thailand and numbers are reducing. This study was investigated the conservation of White Lamphun by cloning technique using ear fibroblasts of male White Lamphun as donor cell. The fusion rate of fibroblasts with enucleated oocytes was 81.7 percent. The development of cloned and parthenogentic activation (PA) embryos were no significant different. The cleavage rate of cloned and PA embryos was 97.5 percent and 82.5 percent, respectively. The percentage of embryos developed to blastocyst stage of cloned and PA were 31.6 percent and 28.6 percent, respectively. The totals of 37 cloned blastocysts were transferred to 26 recipients. Five out of 26 recipients (19.2 percent) were pregnant by ultrasound at 60 days after transfer embryos. Four recipients aborted at days 90, 110, 125 and 135. The recipients naturally delivered one healthy male cloned calf birth weight was 30 kg. Recently, the calf still healthy.