Birth of cloned goats using ear fibroblasts as a donor cell

• Main Authors: Wanwisa Phewsoi(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology) E-mail:rain_ame@hotmail.com, Nucharin Sripunya(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Kanokwan Srirattana(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Anawat Sangmalee(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Kwanrudee Keawmungkun(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Sumeth Imsoonthornruksa(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Chuti Laowtammathron(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Chanchao Lorthongpanich(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Retvin Davahudee(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Mariena Ketudat-Cairns(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology), Rangsun Parnpai(Suranaree University of Technology, Nakhon Ratchasima (Thailand). Institute of Agricultural Technology. School of Biotechnology)
• Format: Conference Proceeding
• Language: Thai
• Published: Bangkok (Thailand) 2008
• Subjects: Cloning; Cytoplasm; Donor cell; Ear fibroblasts; Embryo transfer; Fibroblasts; Goats; Oestrous cycle; Pregnancy; Production

This study was purposed to demonstrate the production of cloned goats using ear fibroblasts as donor cell. The fibroblasts of male and female goat were transferred into enucleated goat oocytes and then fused by electrical stimuli. The fusion rates of both cells were no significantly different (87.2 percent vs 94.2 percent, respectively). Then, the fused oocytes were activated with 7 percent ethanol for 5 min followed by incubated in cycloheximide and cytochalasin D for 5 h. After activation, the reconstructed embryos were cultured in mSOFaa medium for 32-36 h. The 2-8 cell stage embryos were surgically transferred into the oviducts of recipient at the average of 7 embryos/recipient. Ultrasonography at day 30 indicated that 2/15 recipients (13.3 percent) received embryos derived from male fibroblasts were pregnant. However, 16 recipients of cloned embryos derived from female fibroblasts were not pregnant. Both pregnant recipients delivered two healthy male kids by Caesarean section. Both kids were phenotypically and genotypically identical to the donor. Unfortunately, one kid died 32 h postnatal.