Development of polymerase chian reaction (PCR) for the detection and sequence comparison between several isolates of PCV in Thailand

• Main Authors: Tuangthong Patchimasiri, Sudarat Damrong Watanapokin, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr, Worawidh Wajjwalku(Kasetsart Univ., Bangkok (Thailand). Faculty of Veterinary Medicine. Dept. of Veterinary Pathology)
• Format: Conference Proceeding
• Language: Thai
• Published: Bangkok (Thailand) 1999
• Subjects: CIRCOVIRIDAE; DIAGNOSIS; DNA; NUCLEOTIDE SEQUENCE; PCR; POSTWEANING PERIOD; SWINE; THAILAND

There were several cases of pig that had clinical signs and histopathological changes of postweaning multisystemic wasting syndrome (PMWS) submitted to the KPS-Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University and the National Institute of Animal Health. Porcine circovirus (PCV) is a causative agent of PMWS, which is characterized by wasting, respiratory disorder and generalized lymphadenopathy. Sine PCV has never been reported in Thailand. To confirm the presence of PCV, we developed the polymerase chain reaction (PCR) technique for the detection and sequence comparison of several PCV Thai isolates with PCV sequences reported on Genbank database. Total DNA was extracted from tissues of suspected pigs and used as a template for performing PCR with a specific pair of primer. By agarose gel electrophoresis, we were able to see the expected size of the PCR product, which is about 0.5 kb. The PCR products were sequenced and compared with PCV sequences reported on Genebank. In this study, we were able to develop the PCR technique for the detection of PCV and were also confirm the presence of the PCV in Thailand.