Application of PCR diagnosis for bovine leukemia virus (BLV) infection

Bovine leukemia virus (BLV) is an economically important virus for the diary industry because it causes leukemia. Serological diagnosis for BLV has some limitations. In this study, we developed an explored the usefulness of the polymerase chain reaction (PCR) technique for the diagnosis of BLV. Tota...

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Bibliographic Details
Main Authors Theerapol Sirinarumitr, Numdee Saehaeng, Preeda Lertwatcharasarakul, Worawidh Wajjwalku(Kasetsart Univ., Bangkok (Thailand). Faculty of Veterinary Medicine. Dept. of Pathology)
Format Conference Proceeding
LanguageThai
Published Bangkok (Thailand) 1999
Subjects
BOVINE LEUKAEMIA VIRUS
DAIRY CATTLE
IMMUNOLOGY
PCR
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Summary:Bovine leukemia virus (BLV) is an economically important virus for the diary industry because it causes leukemia. Serological diagnosis for BLV has some limitations. In this study, we developed an explored the usefulness of the polymerase chain reaction (PCR) technique for the diagnosis of BLV. Total DNA was extracted from serological positive cattle and negative cattle. A pair of primer was designed to amplify the polymerase gene of BLV. The PCR results had good correlation with the serological results. Howevere, PCR provided the specific and faster result than serological method in the newborn calves receiving colostrum.
Bibliography:TH
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