Effect of ions of cadmium on the enzyme polymorphism in seedlings of maize (Zea mays L.)

• Main Authors: Múdry, P., Trnavská univerzita, Pedagogická fakulta, Katedra biológie, Priemyselná 4, 918 43Trnava (Slovak Republic), Dragúň, M., Trnavská univerzita, Pedagogická fakulta, Katedra biológie, Priemyselná 4, 918 43Trnava (Slovak Republic)
• Format: Conference Proceeding
• Language: Slovak
• Published: Piešťany (Slovak Republic) Slovenské centrum poľnohospodárskeho výskumu, Piešťany (Slovak Republic) 2008
• Subjects: AUTOPOLINIZACION; AUTOPOLLINISATION; CADMIO; CADMIUM; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; GENETIC MARKERS; http://www.fao.org/aos/agrovoc#c_1178; http://www.fao.org/aos/agrovoc#c_15987; http://www.fao.org/aos/agrovoc#c_24030; http://www.fao.org/aos/agrovoc#c_2523; http://www.fao.org/aos/agrovoc#c_33096; http://www.fao.org/aos/agrovoc#c_6945; http://www.fao.org/aos/agrovoc#c_6958; http://www.fao.org/aos/agrovoc#c_8504; ISOENZIMAS; ISOENZYME; ISOENZYMES; MARCADORES GENETICOS; MARQUEUR GENETIQUE; PLANTULAS; PLANTULE; REPUBLICA ESLOVACA; REPUBLIQUE SLOVAQUE; SEEDLINGS; SELF POLLINATION; SLOVAK REPUBLIC; ZEA MAYS
• ISBN: 9788088872887; 808887288X

During 2005 - 2008 we have devoted attention to influence of different concentrations of cadmium ions (0, 15, 60, 240 and 960 micro mol per cubic dm in full Hoagland solutions) on enzyme polymophism of ten enzymes - acid phosphatase (ACP), alcohol dehydrogenase (ADH), CAT-catalase, DIA-diaphorase, GOT-glutamateoxaloacetatetransaminase, isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (PGD), phosphoglucoisomerase (PGI) and phosphoglucomutase (PGM) - in coleoptiles (5 days cultivation), in leaf blades of developed leaves and in the central parts of primary root systems of seedlings (14 days of cultivation) of two self-pollinated maize (Zea mays L.) lines and their single-cross. From analyses and their genetic interpretation resulted: a) the biological material was homogeneous on the basis of enzyme polymorphism, self-pollinated lines had homozygous constitution in each analyzed locus and hybrid had three loci (Acp1: 2/4, Idh2: 4/6 and Pgd1: 2/3.8) with heterozygous constitution, b) ions of cadmium negatively influenced seedling growth and development, concentration of 960 micro mol per cubic dm Cd was toxic and stopped further growth and development of maize seedlings after five days cultivation, c) the coincidence of constitutions in all analyzed loci responsible for enzyme polymorphism expression supported their genotypic, organ and ontogenetic stability at high doses of cadmium ions.