The influence of the type of binary vector and the concentration of agrobacterium inoculum on the expression of reporter gene in transgenic plants

Leaf explants of in vitro grown tobacco plants were transformed with two different binary vectors using Agrobacterium-mediated transformation. These two binary vectors pUN1 and pJL03 differed in bacterial copy number. For each vector, the transformation was performed with two different concentration...

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Bibliographic Details
Main Authors Moravcikova, J, Libantova, J, Nap, J.P, Mlynarova, L
Format Book
LanguageSlovak
Published Piestany (Slovak Republic) Vyskumny ustav rastlinnej vyroby Piestany 2003
Subjects
AGROBACTERIUM TUMEFACIENS
GENETIC TRANSFORMATION
NICOTIANA TABACUM
PLANTAS TRANSGÉNICAS
PLANTE TRANSGÉNIQUE
TRANSFORMACIÓN GENÉTICA
TRANSFORMATION GÉNÉTIQUE
TRANSGENIC PLANTS
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Summary:Leaf explants of in vitro grown tobacco plants were transformed with two different binary vectors using Agrobacterium-mediated transformation. These two binary vectors pUN1 and pJL03 differed in bacterial copy number. For each vector, the transformation was performed with two different concentration of agrobacteria. No obvious differences in transformation efficiency and speed were noted. GUS activity was determined as a measure for the level of GUS gene expression by quantitative fluorometric GUS assays. The measurements showed that the vector type and bacterial density are less influential than anticipated.
Bibliography:2004000095
F30
ISBN:9788088790297
8088790298