Evaluating pollen competition in Douglas-fir using a chloroplast DNA marker
An experiment was conducted to test pollen competition as reflected in the reproductive success of individual pollen lots in two polymixes in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). One polymix contained six pollen lots in equal proportion by weight, the other polymix was formulated usin...
Saved in:
Published in | Forest Genetics (Zvolen) Vol. 6; no. 1 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
1999
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | An experiment was conducted to test pollen competition as reflected in the reproductive success of individual pollen lots in two polymixes in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). One polymix contained six pollen lots in equal proportion by weight, the other polymix was formulated using the same six pollen lots but in inversely proportional amounts to their viability. Pollen lot viability was measured as respiration (oxygen uptake). Reproductive success of individual male parents (pollen lots) was determined following control pollinations using both polymixes separately on six female clones. The paternity analysis was carried out using a chloroplast DNA marker amplified by the polymerase chain reaction (PCR) in total DNA extracts of embryos dissected from mature seed, allowing the identification of the male parent in "polycrossed" seed. Average number of filled seed per cone was not affected by the two polymixes, but the reproductive success of individual males in the polymixes varied greatly. Progeny arrays for both polymixes showed reproductive bias with reproductive success strongly correlated with pollen viability (r2=0.9) |
---|---|
AbstractList | An experiment was conducted to test pollen competition as reflected in the reproductive success of individual pollen lots in two polymixes in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). One polymix contained six pollen lots in equal proportion by weight, the other polymix was formulated using the same six pollen lots but in inversely proportional amounts to their viability. Pollen lot viability was measured as respiration (oxygen uptake). Reproductive success of individual male parents (pollen lots) was determined following control pollinations using both polymixes separately on six female clones. The paternity analysis was carried out using a chloroplast DNA marker amplified by the polymerase chain reaction (PCR) in total DNA extracts of embryos dissected from mature seed, allowing the identification of the male parent in "polycrossed" seed. Average number of filled seed per cone was not affected by the two polymixes, but the reproductive success of individual males in the polymixes varied greatly. Progeny arrays for both polymixes showed reproductive bias with reproductive success strongly correlated with pollen viability (r2=0.9) |
Author | Webber, J.E. (Ministry of Forests, Glyn Road Research Station (Canada)) Stoehr, M.U Mullen, M.C Harrison, D.L.S |
Author_xml | – sequence: 1 fullname: Stoehr, M.U – sequence: 2 fullname: Mullen, M.C – sequence: 3 fullname: Harrison, D.L.S – sequence: 4 fullname: Webber, J.E. (Ministry of Forests, Glyn Road Research Station (Canada)) |
BookMark | eNqFis0KgkAURmdhkJWPENwXEPxLpmWkEQhtauFOLjLa1DhXZsaeP4v2nc0H5zsr5mnSwmN-nKa7MMp4vWSBtY9oJt_nacZ9VpUvVBM6qXsYSSmhoaVhFE46SRqkhoKmXqENO2lgsp8Oob0rMjTO2kFxOcCA5inMhi06VFYEv12z7am8Hc9hh9Rgb6RtrlUSfYk5T_79b2mbOMc |
ContentType | Journal Article |
DBID | FBQ |
DatabaseName | AGRIS |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Forestry |
ExternalDocumentID | SK2000000188 |
GroupedDBID | ALMA_UNASSIGNED_HOLDINGS FBQ P2P |
ID | FETCH-fao_agris_SK20000001882 |
ISSN | 1335-048X |
IngestDate | Tue Nov 07 23:15:51 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-fao_agris_SK20000001882 |
Notes | F30 2000000188 |
ParticipantIDs | fao_agris_SK2000000188 |
PublicationCentury | 1900 |
PublicationDate | (1999) |
PublicationDateYYYYMMDD | 1999-01-01 |
PublicationDate_xml | – year: 1999 text: (1999) |
PublicationDecade | 1990 |
PublicationTitle | Forest Genetics (Zvolen) |
PublicationYear | 1999 |
SSID | ssj0000696348 |
Score | 2.9018652 |
Snippet | An experiment was conducted to test pollen competition as reflected in the reproductive success of individual pollen lots in two polymixes in Douglas-fir... |
SourceID | fao |
SourceType | Publisher |
SubjectTerms | AMENAGEMENT FORESTIER CHLOROPHYLLE CHLOROPHYLLS CLOROFILAS FOREST MANAGEMENT GENETIC MARKERS MARCADORES GENETICOS MARQUEUR GENETIQUE ORDENACION FORESTAL PEUPLEMENT SEMENCIER POLEN POLINIZACION POLLEN POLLINATION POLLINISATION PRODUCCION PRODUCTION PSEUDOTSUGA MENZIESII REPRODUCCION REPRODUCTION RESPIRACION RESPIRATION RODAL SEMILLERO SEED SEED STANDS SEMENCE SEMILLAS |
Title | Evaluating pollen competition in Douglas-fir using a chloroplast DNA marker |
Volume | 6 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3Na8IwGMaDbjB2GfuSfTly2K20VG1rexrOKaKrsKkgu0japiq4dpQ6cH_93iRdmzEH2y5FUkjT_uLbp2-SJwjdeA68Nu3AUk3PIqphE0f1iEHVMPBrxDRBQXNTH3do9SZGf2pOS6VbadbSOvU0_33rupL_UIUy4MpWyf6BbF4pFMBv4AtHIAzHXzHuZFbdbEE5SwCIKeVU-A-xTEYmj9VwmShrnhUgir-AT_T4FYpT5X7YUl7YBJ1EFqlsu044yRypuYkziNDnNzZ-L6UNRmlMF5y2q00KaquVCGOu1i5iW8L3OeTRTXvQcvXJBoNEh-lrHY1dxRU-vnzQXzRC5O1Xm0h5ikmQzxPkGll0XJv7K4AEdj5bF2SL-hwphyHCbqNhqhBLpnJctr51v6_G2KNBnX_i6DXbLqNy02YBrnv3mKfYdAuiC98_La8fFERIZAUxPkQHmfTHLcHxCJVodIz2xH0mmxM0KHBigRNLOPEywhJOzHFigiWcGHBigfMUVbudcbunQjtmZA4AZvKN1CtoJ4ojeoYwoZbfbBIzNHTDoJbuBJ7nhzQwApv5AITnqLK9joufTlyi_eLpX6HdEP4HtAqiKvWu-aP7AMZOJ6w |
link.rule.ids | 786 |
linkProvider | FAO Food and Agriculture Organization of the United Nations |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Evaluating+pollen+competition+in+Douglas-fir+using+a+chloroplast+DNA+marker&rft.jtitle=Forest+Genetics+%28Zvolen%29&rft.au=Stoehr%2C+M.U&rft.au=Mullen%2C+M.C&rft.au=Harrison%2C+D.L.S&rft.au=Webber%2C+J.E.+%28Ministry+of+Forests%2C+Glyn+Road+Research+Station+%28Canada%29%29&rft.date=1999-01-01&rft.issn=1335-048X&rft.volume=6&rft.issue=1&rft.externalDocID=SK2000000188 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1335-048X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1335-048X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1335-048X&client=summon |